8OPZ
Crystal structure of a tailspike depolymerase (APK16_gp47) from Acinetobacter phage APK16
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-12 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97 |
| Spacegroup name | H 3 |
| Unit cell lengths | 88.045, 88.045, 254.969 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 84.990 - 1.500 |
| R-factor | 0.17667 |
| Rwork | 0.175 |
| R-free | 0.21471 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 3.078 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | CRANK2 |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.990 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.250 | 5.952 |
| Rmeas | 0.256 | 6.098 |
| Rpim | 0.057 | 1.321 |
| Total number of observations | 2443418 | 120018 |
| Number of reflections | 117005 | 5743 |
| <I/σ(I)> | 4.7 | 0.2 |
| Completeness [%] | 99.8 | |
| Redundancy | 20.9 | 20.9 |
| CC(1/2) | 0.996 | 0.482 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 288 | 100 mM Tris pH 8.5, 2.0M Ammonium sulfate |
