8OPR
Structure of the EA1 surface layer of Bacillus anthracis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-06-10 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.98112 |
| Spacegroup name | P 1 |
| Unit cell lengths | 72.927, 74.297, 87.649 |
| Unit cell angles | 107.85, 101.14, 112.42 |
Refinement procedure
| Resolution | 63.370 - 1.811 |
| R-factor | 0.2056 |
| Rwork | 0.204 |
| R-free | 0.24340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.030 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4 (3-FEB-2022)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.350 | 2.038 |
| High resolution limit [Å] | 1.811 | 1.811 |
| Rmerge | 0.550 | |
| Number of reflections | 73143 | 3659 |
| <I/σ(I)> | 9.1 | |
| Completeness [%] | 99.0 | |
| Redundancy | 3.1 | |
| CC(1/2) | 0.997 | 0.689 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 2 M ammonium sulfate and 0.1 M sodium acetate pH 5.0 |
