8ONJ
Crystal structure of D-amino acid aminotransferase from Aminobacterium colombiense point mutant R88L
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-17 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54184 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.791, 88.553, 100.663 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 66.490 - 1.800 |
| R-factor | 0.19069 |
| Rwork | 0.189 |
| R-free | 0.21900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.719 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.7) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.550 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.093 | 1.108 |
| Rmeas | 0.102 | 1.345 |
| Rpim | 0.041 | 0.746 |
| Total number of observations | 306052 | 8483 |
| Number of reflections | 50878 | 2853 |
| <I/σ(I)> | 14.3 | 1 |
| Completeness [%] | 99.6 | |
| Redundancy | 6 | 3 |
| CC(1/2) | 0.998 | 0.389 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 288 | 0.2 M Sodium nitrate, 0.1 M Bis-Tris propane pH 6.5, 20 % w/v PEG 3350 |
