8ONF
Crystal structure of Bdellovibrio bacteriovorus Bd2439 fibre C-terminal domains with ethylene glycol
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-06-06 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 84.368, 161.278, 52.067 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.340 - 1.530 |
R-factor | 0.1653 |
Rwork | 0.164 |
R-free | 0.18510 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.863 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 84.370 | 1.550 |
High resolution limit [Å] | 1.530 | 1.530 |
Rmerge | 0.062 | 0.653 |
Rmeas | 0.073 | 0.842 |
Rpim | 0.037 | 0.526 |
Total number of observations | 341050 | 8323 |
Number of reflections | 104740 | 4168 |
<I/σ(I)> | 11.2 | 1.1 |
Completeness [%] | 96.9 | |
Redundancy | 3.3 | 2 |
CC(1/2) | 0.998 | 0.638 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.1 M Sodium chloride 0.1 M Sodium formate 0.1 M Bis-Tris propane 8.5 6.25 % w/v PEG 3350 6.25%w/v PEG 4000 6.25%w/v PEG 2000 6.25%w/v PEG 5000 MME |