8OND

Structure of the C-terminal domains of the Bdellovibrio bacteriovorus Bd2133 fibre

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-30
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	P 63
Unit cell lengths	65.308, 65.308, 319.187
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.140 - 2.500
R-factor	0.2017
Rwork	0.200
R-free	0.22790
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.976
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.690	2.600
High resolution limit [Å]	2.500	2.500
Number of reflections	26645	2990
<I/σ(I)>	24.5
Completeness [%]	100.0
Redundancy	42.4
CC(1/2)	1.000	0.896

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.03 M sodium fluoride, 0.03M sodium bromide, 0.03M sodium iodide, 0.1 M imidazole/MES pH 6.5, 20 % v/v glycerol, 10 % w/v PEG 4000