8ONC

Structure of the C-terminal beta helix domain of the Bdellovibrio bacteriovorus Bd3182 fibre

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-28
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97
Spacegroup name	I 1 2 1
Unit cell lengths	111.457, 48.153, 172.204
Unit cell angles	90.00, 107.11, 90.00

Refinement procedure

Resolution	79.410 - 2.000
R-factor	0.1776
Rwork	0.176
R-free	0.20340
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.670
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	79.410	2.050
High resolution limit [Å]	2.000	2.000
Number of reflections	59598	4316
<I/σ(I)>	9
Completeness [%]	100.0
Redundancy	6.1
CC(1/2)	0.997	0.879

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	1.0 M sodium citrate tribasic dihydrate, 0.1 M HEPES pH 7.