8ONB
Structure of the C-terminal beta helix domain of the Bdellovibrio bacteriovorus Bd3182 fibre
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-01-27 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.561, 48.397, 92.964 |
| Unit cell angles | 90.00, 102.98, 90.00 |
Refinement procedure
| Resolution | 84.350 - 1.120 |
| R-factor | 0.15 |
| Rwork | 0.149 |
| R-free | 0.17230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.189 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.350 | 1.241 |
| High resolution limit [Å] | 1.120 | 1.120 |
| Number of reflections | 176672 | 8836 |
| <I/σ(I)> | 16.4 | |
| Completeness [%] | 61.3 | |
| Redundancy | 6.8 | |
| CC(1/2) | 0.999 | 0.706 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.1 M magnesium acetate tetrahydrate, 0.1 M MES pH 6.5, 10 % w/v PEG 10000 |
