8ON5
Structure of the tail fibre from an extracellular contractile injection system from Photorhabdus bacteria
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-01-28 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.97951 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.458, 53.536, 220.660 |
| Unit cell angles | 90.00, 95.65, 90.00 |
Refinement procedure
| Resolution | 60.030 - 2.300 |
| R-factor | 0.18191 |
| Rwork | 0.179 |
| R-free | 0.23582 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.482 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0403) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.030 | 2.350 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.103 | 0.669 |
| Rmeas | 0.113 | 0.735 |
| Rpim | 0.046 | 0.301 |
| Total number of observations | 26868 | |
| Number of reflections | 70537 | 4480 |
| <I/σ(I)> | 9.7 | 2.3 |
| Completeness [%] | 100.0 | |
| Redundancy | 6.2 | 6 |
| CC(1/2) | 0.997 | 0.941 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 290 | 0.02 M CaCl2 dihydrate, 0.1M Na acetate pH 4.6, 30% MPD |
