8ON4

Structure of the C-terminal domains of the Bdellovibrio bacteriovorus Bd1334 fibre

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-30
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	H 3
Unit cell lengths	82.940, 82.940, 67.670
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.470 - 1.410
R-factor	0.1725
Rwork	0.171
R-free	0.19890
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.022
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.470	1.450
High resolution limit [Å]	1.410	1.410
Number of reflections	30502	1320
<I/σ(I)>	22.2
Completeness [%]	91.1
Redundancy	9
CC(1/2)	1.000	0.395

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.2 M lithium chloride, 0.1 M MES pH 6.0, 20 % w/v PEG 6000