8OML

Crystal structure of Bdellovibrio bacteriovorus Bd1334 C-terminal domains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-27
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	196.738, 81.863, 58.850
Unit cell angles	90.00, 98.11, 90.00

Refinement procedure

Resolution	47.390 - 2.560
R-factor	0.2172
Rwork	0.215
R-free	0.25670
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.187
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	97.390	2.900
High resolution limit [Å]	2.560	2.560
Number of reflections	14282	714
<I/σ(I)>	8.5
Completeness [%]	47.5
Redundancy	3.5
CC(1/2)	0.998	0.587

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.03 M sodium nitrate, 0.03 M sodium phosphate dibasic, 0.03 M ammonium sulphate, 0.1 M Imidazole/MES pH 6.5, 20 % v/v PEG 500 MME, 10 % w/v PEG 20000