8OLZ
Structure of Oceanobacillus iheyensis group II intron in the presence of K+, Mg2+, 5'-exon, and intronistat B after 2h30 soaking
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-1 |
Synchrotron site | ESRF |
Beamline | MASSIF-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-04-14 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.96 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 87.672, 93.968, 223.433 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.840 - 3.310 |
R-factor | 0.19579 |
Rwork | 0.192 |
R-free | 0.27320 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.880 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0403) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.840 | 3.400 |
High resolution limit [Å] | 3.300 | 3.300 |
Number of reflections | 28270 | 2786 |
<I/σ(I)> | 4.57 | |
Completeness [%] | 99.5 | |
Redundancy | 6.5 | |
CC(1/2) | 0.990 | 0.320 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 303.15 | 100 mM magnesium acetate, 150 mM potassium chloride, 10 mM lithium chloride, 50 mM HEPES sodium, pH 7.0, 4% PEG8000 |