8OLV
Structure of Oceanobacillus iheyensis group II intron in the presence of K+, Mg2+ and ARN25850
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-05-09 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 90.012, 94.722, 225.042 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.420 - 2.810 |
| R-factor | 0.19785 |
| Rwork | 0.196 |
| R-free | 0.23740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.819 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.420 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.110 | |
| Number of reflections | 46958 | 4256 |
| <I/σ(I)> | 11.97 | |
| Completeness [%] | 97.9 | |
| Redundancy | 12.4 | |
| CC(1/2) | 1.000 | 0.083 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 303.15 | 100 mM magnesium acetate, 100 mM potassium chloride, 10 mM lithium chloride, 50 mM HEPES sodium, pH 7.0, 3.5% PEG8000 |
