8OLV
Structure of Oceanobacillus iheyensis group II intron in the presence of K+, Mg2+ and ARN25850
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-05-09 |
Detector | DECTRIS EIGER2 X 9M |
Wavelength(s) | 0.92 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 90.012, 94.722, 225.042 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.420 - 2.810 |
R-factor | 0.19785 |
Rwork | 0.196 |
R-free | 0.23740 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.819 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.420 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.110 | |
Number of reflections | 46958 | 4256 |
<I/σ(I)> | 11.97 | |
Completeness [%] | 97.9 | |
Redundancy | 12.4 | |
CC(1/2) | 1.000 | 0.083 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 303.15 | 100 mM magnesium acetate, 100 mM potassium chloride, 10 mM lithium chloride, 50 mM HEPES sodium, pH 7.0, 3.5% PEG8000 |