8OLS
Structure of Oceanobacillus iheyensis group II intron in the presence of K+, Mg2+ and intronistat B
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-1 |
Synchrotron site | ESRF |
Beamline | MASSIF-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-09-01 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.96 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 89.016, 94.961, 223.855 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.690 - 3.000 |
R-factor | 0.19194 |
Rwork | 0.189 |
R-free | 0.24241 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 2.310 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0411) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.700 | 3.100 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.044 | |
Number of reflections | 38577 | 3799 |
<I/σ(I)> | 18.58 | |
Completeness [%] | 99.3 | |
Redundancy | 5.4 | |
CC(1/2) | 1.000 | 0.350 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 303.15 | 100 mM magnesium acetate, 200 mM potassium chloride, 10 mM lithium chloride, 50 mM HEPES sodium, pH 7.0, 3% PEG8000 |