8OLJ

Crystal structure of Archaeoglobus fulgidus AfAgo-N protein representing N-L1-L2 domains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-23
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.97970
Spacegroup name	P 32 2 1
Unit cell lengths	75.264, 75.264, 94.722
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	53.700 - 1.400
R-factor	0.1479
Rwork	0.145
R-free	0.17772
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.610
Data reduction software	XDS (VERSION Jan 26, 2018 BUILT=20180126)
Data scaling software	Aimless (version 0.7.2)
Phasing software	MOLREP (Vers 11.6.04)
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	94.720	1.420
High resolution limit [Å]	1.400	1.400
Rmerge	0.047	0.908
Rmeas	0.049	0.967
Rpim	0.016	0.331
Number of reflections	61624	3045
<I/σ(I)>	23.8	2.3
Completeness [%]	100.0	100
Redundancy	9.7	8.4
CC(1/2)	0.999	0.810

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		280	Protein concentration 4.1 mg/ml. Reservoir: TrisHCl pH 8.0 0.05 M; iPrOH 27%; ammonium acetate 0.11 M; Bicine pH 9.0 0.05 M. Cryo-protection: short wash in 0.16 M Ammonium acetate; 0.08 M TrisHCl pH 8.5; 24% iPrOH; Glycerol 20%