8OLH
Y345F Variant of Dye Type Peroxidase Aa (DtpAa) from Streptomyces lividans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-04-23 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.022, 67.788, 73.024 |
| Unit cell angles | 90.00, 105.66, 90.00 |
Refinement procedure
| Resolution | 70.320 - 1.230 |
| R-factor | 0.13444 |
| Rwork | 0.133 |
| R-free | 0.15875 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.748 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0405) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.320 | 1.250 |
| High resolution limit [Å] | 1.230 | 1.230 |
| Rmerge | 0.093 | 0.944 |
| Rmeas | 0.116 | 1.267 |
| Rpim | 0.070 | 0.836 |
| Number of reflections | 191400 | 7299 |
| <I/σ(I)> | 8.2 | |
| Completeness [%] | 97.8 | |
| Redundancy | 4.8 | 2.8 |
| CC(1/2) | 0.996 | 0.357 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 1:1 RATIO OF 15 MG/ML PROTEIN SOLUTION WITH A PRECIPITANT SOLUTION CONTAINING 20% PEG 6000, 100 MM HEPES PH 7.0 |
