8OLD

Crystal structure of Archaeoglobus fulgidus AfAgo-N protein representing N-L1-L2 domains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-04
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.97680
Spacegroup name	P 1
Unit cell lengths	42.033, 57.843, 61.622
Unit cell angles	73.94, 89.56, 89.75

Refinement procedure

Resolution	47.630 - 1.850
R-factor	0.18299
Rwork	0.179
R-free	0.21819
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.527
Data reduction software	XDS (VERSION Jun 17, 2015)
Data scaling software	SCALA (3.3.22 : 21/07/13)
Phasing software	MOLREP (Vers 9.2.10; 30.11.2005)
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.590	1.950
High resolution limit [Å]	1.850	1.850
Rmerge	0.033
Rmeas	0.044	0.696
Rpim	0.033	0.492
Number of reflections	44415	6503
<I/σ(I)>	15.9
Completeness [%]	93.2	93.5
Redundancy	3.6	3.6
CC(1/2)	0.999	0.817

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	280	NaCl 0.012 M; KCl 0.08 M; Sodium Cacodylate pH 6.0 0.04 M; MPD 50% ;spermin 0.012 M; 9 mg/ml protein concentration