8OKY
Crystal structure of D-ProB26-DTriA analogue of human insulin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2009-02-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0723 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 22.909, 28.261, 59.373 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.380 - 1.170 |
| R-factor | 0.13579 |
| Rwork | 0.134 |
| R-free | 0.16635 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.978 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0405) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.000 | 1.180 |
| High resolution limit [Å] | 1.160 | 1.160 |
| Rmerge | 0.046 | 0.217 |
| Number of reflections | 12934 | 424 |
| <I/σ(I)> | 20.6 | |
| Completeness [%] | 97.7 | |
| Redundancy | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 293 | 0.3 M Tris/HCl pH 8.2, 0.6 M Na2SO4, 0.6 mM ZnAc |
