8OKF
WD repeat containing protein 5 (WDR5)- PER2 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-03 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97630 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 78.152, 78.152, 101.748 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.110 - 1.850 |
| R-factor | 0.16684 |
| Rwork | 0.165 |
| R-free | 0.20070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.647 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.150 | 1.890 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 27636 | 1653 |
| <I/σ(I)> | 18.3 | |
| Completeness [%] | 100.0 | |
| Redundancy | 14.2 | |
| CC(1/2) | 0.999 | 0.955 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 0.1 M Tris pH 8.0, 50 mM sodium chloride, 13% PEG8000 |
