8OK6
Variant Surface Glycoprotein VSG11 two monomers
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-06 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 76.244, 76.244, 212.257 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.270 - 1.750 |
| R-factor | 0.2023 |
| Rwork | 0.201 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.176 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | SHELX |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.270 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 72926 | 7093 |
| <I/σ(I)> | 19.09 | 0.98 |
| Completeness [%] | 99.8 | |
| Redundancy | 19 | |
| CC(1/2) | 1.000 | 0.348 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 296 | 2.0 M AmmoniumSulfate 100 mM Sodium acetate |
