8OK5
Variant Surface Glycoprotein VSG11 monomer with iodine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-06 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 75.426, 75.426, 106.441 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.260 - 1.270 |
| R-factor | 0.2076 |
| Rwork | 0.206 |
| R-free | 0.23010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.234 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2-4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.260 | 1.315 |
| High resolution limit [Å] | 1.270 | 1.270 |
| Number of reflections | 93126 | 9185 |
| <I/σ(I)> | 14.54 | 1.27 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.7 | 9.4 |
| CC(1/2) | 0.999 | 0.471 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 296 | 1.7 M NaKTartrate 100 mM Tris/HCl |
