8OJQ
Arabidopsis thaliana Phosphoenolpyruvate carboxylase PPC1 T778 mutant with bound phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-05-27 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.981 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 128.000, 159.840, 141.920 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.189 - 3.049 |
R-factor | 0.206582212729 |
Rwork | 0.205 |
R-free | 0.24022 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.818 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.189 | 3.150 |
High resolution limit [Å] | 3.049 | 3.049 |
Rmerge | 0.092 | 2.493 |
Number of reflections | 55452 | 5360 |
<I/σ(I)> | 14.89 | 0.88 |
Completeness [%] | 99.7 | 96.6 |
Redundancy | 14 | |
CC(1/2) | 0.999 | 0.444 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.09M Sodium nitrate, 0.09 Sodium phosphate dibasic, 0.09M Ammonium sulfate, 0.1M MES monohydrate pH6.5, 20% v/v Ethylene glycol; 10 % w/v PEG 8K |