8OJN

Structure of the C-terminal beta helix domain of the Bdellovibrio bacteriovorus Bd3182 fibre

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-13
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97
Spacegroup name	P 1 21 1
Unit cell lengths	93.219, 48.133, 95.595
Unit cell angles	90.00, 96.69, 90.00

Refinement procedure

Resolution	92.580 - 2.510
R-factor	0.1962
Rwork	0.194
R-free	0.24810
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.821
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	92.968	2.210
High resolution limit [Å]	2.173	2.173
Number of reflections	39829	1511
<I/σ(I)>	9.1
Completeness [%]	87.6	67.3
Redundancy	6.3
CC(1/2)	0.994	0.349

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291.15	0.03 M sodium nitrate, 0.03 M sodium phosphate dibasic, 0.03 M ammonium sulphate, 0.1 M imidazole/MES pH 6.5, 20 % v/v PEG 500 MME, 10 % w/v PEG 20000