8OJE
Arabidopsis thaliana Phosphoenolpyruvate carboxylase PPC1 in complex with L-malate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-16 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.981 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 116.080, 352.200, 176.040 |
| Unit cell angles | 90.00, 101.84, 90.00 |
Refinement procedure
| Resolution | 48.925 - 3.142 |
| R-factor | 0.20393187438 |
| Rwork | 0.202 |
| R-free | 0.23976 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.837 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.330 |
| High resolution limit [Å] | 3.142 | 3.143 |
| Rmerge | 0.292 | 2.102 |
| Number of reflections | 237667 | 36978 |
| <I/σ(I)> | 6.94 | 0.89 |
| Completeness [%] | 99.3 | 96.1 |
| Redundancy | 7.11 | 6.42 |
| CC(1/2) | 0.993 | 0.352 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 293 | 30%PEG400, 0.1 M Hepes Sodium Salt pH 7.5, 200mM Magnesium Chloride |
