8OJ2

Crystal structure of the DNA binding domain of M. polymorpha Auxin Response Factor 2 (MpARF2) in complex with protomor-like sequence IR7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0722
Spacegroup name	I 21 21 21
Unit cell lengths	79.622, 79.682, 146.297
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.320 - 2.560
R-factor	0.2221
Rwork	0.220
R-free	0.26600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.641
Data reduction software	autoPROC (1.0.5)
Data scaling software	autoPROC (1.0.5)
Phasing software	PHENIX (1.19.2_4158)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.148	2.747
High resolution limit [Å]	2.558	2.558
Rmerge	0.123	2.670
Rmeas	0.129
Number of reflections	12755	638
<I/σ(I)>	12.8
Completeness [%]	91.3	41.8
Redundancy	12.3
CC(1/2)	0.995	0.448

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	1 ul of protein at 4.6mg/ml in 15mM Hepes pH7.5, 150mM NaCl, 20mM DTT were mixed with 1 ul reservoir (0.2M Ammonium sulfate, 0.1M MES pH6.5, 24% PEG 3350) and equilibrated against 80 ul reservoir