8OIK

D-PHAT domain (NTD) of human SAMD4A

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.968, 0.98,
Spacegroup name	C 2 2 21
Unit cell lengths	81.829, 142.786, 137.096
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.450 - 1.620
R-factor	0.2073
Rwork	0.207
R-free	0.22090
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	0.947
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.050	1.700
High resolution limit [Å]	1.620	1.620
Rmerge	0.060
Rmeas	0.062
Number of reflections	89788	4488
<I/σ(I)>	19.2
Completeness [%]	95.2
Redundancy	13.8
CC(1/2)	0.999	0.340

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	0.1 M sodium citrate pH 5.6, 8% jeffamine M-600 pH 7