8OI5

Crystal structure of the Candida albicans 80S ribosome in complex with Paromomycin (2mM)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	173
Detector technology	PIXEL
Collection date	2022-05-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	297.300, 292.540, 442.980
Unit cell angles	90.00, 99.37, 90.00

Refinement procedure

Resolution	163.000 - 2.900
R-factor	0.2573
Rwork	0.257
R-free	0.28330
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.120
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.19rc4_4035)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	163.000	163.000	2.950
High resolution limit [Å]	2.900	50.000	2.900
Rmerge	0.556	0.036	3.280
Rmeas	0.599	0.039	3.526
Rpim	0.221
Number of reflections	1631597	263	81692
<I/σ(I)>	4.8
Completeness [%]	99.2
Redundancy	21.7
CC(1/2)	0.986	0.998	0.069

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277.15	PEG 20K, KSCN, Mg Acetate, K - acetate, Bis-Tris-Acetate, Glycerol, Spermidine, phyllanthoside