8OG7

Crystal structure of human DCAF1 WD40 repeats (Q1250L) in complex with compound 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.000
Spacegroup name	P 61 2 2
Unit cell lengths	81.750, 81.750, 235.633
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	52.640 - 2.640
R-factor	0.16006
Rwork	0.158
R-free	0.20598
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.916
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.640	2.770
High resolution limit [Å]	2.640	2.640
Number of reflections	13467	1764
<I/σ(I)>	11.1
Completeness [%]	94.4
Redundancy	13.3
CC(1/2)	0.994	0.752

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.1M MES pH6.5, 2.13M Lithium acetate