8OG7
Crystal structure of human DCAF1 WD40 repeats (Q1250L) in complex with compound 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 81.750, 81.750, 235.633 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 52.640 - 2.640 |
| R-factor | 0.16006 |
| Rwork | 0.158 |
| R-free | 0.20598 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.916 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.640 | 2.770 |
| High resolution limit [Å] | 2.640 | 2.640 |
| Number of reflections | 13467 | 1764 |
| <I/σ(I)> | 11.1 | |
| Completeness [%] | 94.4 | |
| Redundancy | 13.3 | |
| CC(1/2) | 0.994 | 0.752 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1M MES pH6.5, 2.13M Lithium acetate |
