8OG4
Exostosin-like 3 UDP complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-12-08 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9212 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 121.617, 121.617, 259.537 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.520 - 2.100 |
| R-factor | 0.1941 |
| Rwork | 0.192 |
| R-free | 0.23080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.286 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.18rc1_3769) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.160 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rpim | 0.048 | 0.986 |
| Number of reflections | 129790 | 6395 |
| <I/σ(I)> | 7.4 | |
| Completeness [%] | 100.0 | |
| Redundancy | 12.4 | |
| CC(1/2) | 0.998 | 0.255 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 12.5% (w/v) PEG3350, 0.1 M malic acid, pH 7.0. Soaked overnight in 15% PEG 3350, 0.1 M malic acid, pH 7.0, 5 mM UDP and 5 mM MnCl2. |
