8OFU
Human adenovirus type 53 fiber-knob protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-14 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.91188 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 206.314, 60.526, 93.837 |
| Unit cell angles | 90.00, 91.80, 90.00 |
Refinement procedure
| Resolution | 68.420 - 1.610 |
| R-factor | 0.19231 |
| Rwork | 0.191 |
| R-free | 0.21275 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.562 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.489 | 1.633 |
| High resolution limit [Å] | 1.606 | 1.606 |
| Rmerge | 0.099 | 1.569 |
| Rmeas | 0.116 | 1.853 |
| Rpim | 0.059 | 0.972 |
| Number of reflections | 150644 | 7529 |
| <I/σ(I)> | 8.7 | 0.8 |
| Completeness [%] | 99.7 | 99.4 |
| Redundancy | 3.7 | 3.5 |
| CC(1/2) | 0.998 | 0.334 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M potassium thiocyanate, 20 % w/v PEG 3350, pH unadjusted |
