8OFR

Human adenovirus type 25 fiber-knob protein complexed with sialic acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.88561
Spacegroup name	P 21 21 21
Unit cell lengths	127.562, 179.321, 207.897
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	64.640 - 2.510
R-factor	0.22175
Rwork	0.221
R-free	0.23921
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.303
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.640	2.580
High resolution limit [Å]	2.510	2.510
Rmerge	0.218	2.268
Rmeas	0.234	2.427
Rpim	0.084	0.860
Total number of observations		93395
Number of reflections	162833	11926
<I/σ(I)>	6	0.9
Completeness [%]	100.0
Redundancy	7.7	7.8
CC(1/2)	0.988	0.451

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	291	0.1 M SPG buffer, 25 % w/v PEG 1500