8OFQ

Human adenovirus type 25 fiber-knob protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-17
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9795
Spacegroup name	H 3
Unit cell lengths	126.552, 126.552, 251.444
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	82.610 - 1.600
R-factor	0.17342
Rwork	0.172
R-free	0.19791
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.499
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	82.620	1.640
High resolution limit [Å]	1.600	1.600
Rmerge	0.071	1.284
Rmeas	0.078	1.418
Rpim	0.045	0.815
Number of reflections	198233	14640
<I/σ(I)>	11.2	1.1
Completeness [%]	100.0	100
Redundancy	5.8	5.6
CC(1/2)	0.999	0.484

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	0.1 M SPG buffer, 25 % w/v PEG 1500