8OFP
Human adenovirus type 24 fiber-knob protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-17 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.259, 99.619, 113.549 |
| Unit cell angles | 90.00, 100.26, 90.00 |
Refinement procedure
| Resolution | 55.870 - 3.100 |
| R-factor | 0.23903 |
| Rwork | 0.234 |
| R-free | 0.33672 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.371 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.960 | 2.980 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.150 | 1.117 |
| Rmeas | 0.175 | 1.296 |
| Rpim | 0.089 | 0.649 |
| Total number of observations | 96302 | 7260 |
| Number of reflections | 25128 | 1852 |
| <I/σ(I)> | 4.4 | 1 |
| Completeness [%] | 100.0 | |
| Redundancy | 3.8 | 3.9 |
| CC(1/2) | 0.973 | 0.652 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 291 | 0.1 M MMT Buffer, 25% PEG 1500 |
