8OFN

Structure of the yellow fever virus (Asibi strain) dimeric envelope protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.
Spacegroup name	P 43 21 2
Unit cell lengths	92.540, 92.540, 308.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 3.480
R-factor	0.256
Rwork	0.255
R-free	0.27300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.860
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	BUSTER

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.300	3.810
High resolution limit [Å]	3.480	3.480
Rmerge	0.117	2.200
Rmeas	0.121	2.280
Rpim	0.030	0.600
Number of reflections	17868	568
<I/σ(I)>	13.1
Completeness [%]	93.4
Redundancy	15.5
CC(1/2)	0.990	0.660

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	1.26M ammonium sulfate, 0.1M HEPES pH 7.5