8OEQ

Crystal structure of the Candida albicans 80S ribosome in complex with Paromomycin (250uM)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	173
Detector technology	PIXEL
Collection date	2022-04-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	298.320, 293.370, 451.080
Unit cell angles	90.00, 100.16, 90.00

Refinement procedure

Resolution	177.030 - 3.300
R-factor	0.2542
Rwork	0.254
R-free	0.29750
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	1.012
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.19rc4_4035)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	177.030	177.030	3.350
High resolution limit [Å]	3.300	50.000	3.300
Rmerge	0.695	0.051	3.382
Rmeas	0.721	0.054	3.503
Rpim	0.189
Number of reflections	1134400	286	50049
<I/σ(I)>	4.36
Completeness [%]	99.3
Redundancy	14.4
CC(1/2)	0.992	0.997	0.052

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277.15	PEG 20K, KSCN, Mg Acetate, K - acetate, Bis-Tris-Acetate, Glycerol, Spermidine, phyllanthoside