8KHS
the structure of a glutamine amidotransferase DnfC from Alcaligenes sp.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 66.176, 78.403, 86.324 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.201 - 2.100 |
| R-factor | 0.2454 |
| Rwork | 0.244 |
| R-free | 0.28130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.634 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.210 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.069 | 1.081 |
| Rmeas | 0.072 | 1.147 |
| Rpim | 0.021 | 0.374 |
| Number of reflections | 13421 | 1069 |
| <I/σ(I)> | 18.5 | |
| Completeness [%] | 99.8 | 98.6 |
| Redundancy | 12 | |
| CC(1/2) | 0.999 | 0.913 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 4% Tacsimate pH 5.0, 10% PEG 3350 |
