8KH6

Crystal structure of FGFR4 kinase domain with 8r

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2023-01-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	41.780, 61.180, 60.720
Unit cell angles	90.00, 97.76, 90.00

Refinement procedure

Resolution	27.270 - 1.620
R-factor	0.205
Rwork	0.204
R-free	0.22760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ybo
RMSD bond length	0.007
RMSD bond angle	0.966
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.270	7.240
High resolution limit [Å]	1.620	1.620
Number of reflections	37228	37169
<I/σ(I)>	14.8
Completeness [%]	96.2
Redundancy	6.2
CC(1/2)	0.999	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1 M Bis-Tris (pH 4.5), 0.2 M Li2SO4, 16-19% PEG 3350