8KGZ
Crystal structure of single-chain Fv antibody against antigen peptide from SARS-CoV2 S-spike protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-01-01 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.210, 77.753, 61.346 |
| Unit cell angles | 90.00, 112.25, 90.00 |
Refinement procedure
| Resolution | 55.790 - 2.210 |
| R-factor | 0.27129 |
| Rwork | 0.269 |
| R-free | 0.32114 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.930 |
| Data reduction software | HKL-3000 |
| Data scaling software | DIALS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.750 | 2.270 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Number of reflections | 179188 | 26361 |
| <I/σ(I)> | 4.1 | |
| Completeness [%] | 100.0 | |
| Redundancy | 6.5 | 6.4 |
| CC(1/2) | 0.882 | 0.882 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 25% PEG 3350, 0.1M HEPES pH7.5 |
