8KGT
Structure of African swine fever virus topoisomerase II in complex with dsDNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2022-10-27 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 85.234, 85.234, 209.809 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.790 - 2.300 |
| R-factor | 0.19559 |
| Rwork | 0.193 |
| R-free | 0.24593 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.135 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (5.8.0405) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.094 | 0.945 |
| Rmeas | 0.159 | |
| Rpim | 0.055 | |
| Number of reflections | 20799 | 996 |
| <I/σ(I)> | 14.22 | 2.24 |
| Completeness [%] | 99.4 | 99.4 |
| Redundancy | 8.2 | 7.7 |
| CC(1/2) | 0.999 | 0.629 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 21% PEG 3350, 100 mM Hepes (pH 7.2) and 200 mM Li2SO4 |
