8KGB
SlNDPS1-AtcPT4 Chimera complexed with GSPP, Mg2+, and IPP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2022-12-17 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.540, 126.710, 72.690 |
| Unit cell angles | 90.00, 90.25, 90.00 |
Refinement procedure
| Resolution | 47.810 - 2.320 |
| R-factor | 0.1905 |
| Rwork | 0.188 |
| R-free | 0.23518 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.347 |
| Data reduction software | DIALS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.460 |
| High resolution limit [Å] | 2.320 | 2.320 |
| Rmeas | 0.109 | 1.390 |
| Number of reflections | 275956 | 44464 |
| <I/σ(I)> | 11.17 | 1.22 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.9 | 5.9 |
| CC(1/2) | 0.998 | 0.547 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | PEG3350, malonate acid |
