8KG2

Crystal structure of p97-N/D1 hexamer in complex with FAF1-UBX domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-12-19
Detector	ADSC QUANTUM 210r
Wavelength(s)	1.0000
Spacegroup name	P 1
Unit cell lengths	106.665, 134.484, 148.223
Unit cell angles	71.52, 80.87, 87.53

Refinement procedure

Resolution	48.800 - 3.100
R-factor	0.20016
Rwork	0.198
R-free	0.24626
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.560
Data reduction software	XDS
Data scaling software	Aimless (1.12.12)
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.800	3.150
High resolution limit [Å]	3.100	3.100
Rmeas	0.088	0.839
Number of reflections	150419	7512
<I/σ(I)>	7.7	1.2
Completeness [%]	97.4
Redundancy	3.9
CC(1/2)	0.998	0.704

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	295	0.2M sodium acetate, 0.1M sodium citrate (pH 5.6), 5% polyethylene glycol 4000