8KFS

Crystal structure of ZmMOC1/nicked Holliday junction complex at ground state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-05-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.978
Spacegroup name	P 1 21 1
Unit cell lengths	54.263, 78.028, 63.456
Unit cell angles	90.00, 98.11, 90.00

Refinement procedure

Resolution	19.510 - 2.150
R-factor	0.2043
Rwork	0.203
R-free	0.22750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6jrg
RMSD bond length	0.008
RMSD bond angle	0.882
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.21rc1_4958)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.310
High resolution limit [Å]	2.150	2.150
Rmerge	0.143	0.763
Number of reflections	28620	5689
<I/σ(I)>	13.02	2.5
Completeness [%]	97.9	99.7
Redundancy	6.8
CC(1/2)		0.837

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	6% Isopropyl alcohol, 0.1 M Sodium acetate pH5.0, 35% Polyethylene glycol monomethyl ether 550