8KFR

Crystal structure of ZmMOC1/nicked Holliday junction/Ca2+ complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.978
Spacegroup name	P 1 21 1
Unit cell lengths	53.980, 77.600, 63.280
Unit cell angles	90.00, 98.05, 90.00

Refinement procedure

Resolution	43.810 - 2.100
R-factor	0.2031
Rwork	0.201
R-free	0.23970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6jrg
RMSD bond length	0.008
RMSD bond angle	0.948
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.21rc1_4958)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.660	2.150
High resolution limit [Å]	2.100	2.100
Rmerge	0.153	1.053
Number of reflections	30203	2212
<I/σ(I)>	10.5	2.4
Completeness [%]	99.8
Redundancy	6.8
CC(1/2)	0.995	0.995

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		300	0.05 M Calcium chloride, HEPES 7.0, 40% Polyethylene glycol 200