8KFD

Ferritin drug carrier(FDC) for encapsulated platinum (IV) prodrug for esophageal squamous cell carcinoma targeted therapy

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97915
Spacegroup name	F 4 3 2
Unit cell lengths	183.450, 183.450, 183.450
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.300 - 1.800
R-factor	0.154
Rwork	0.153
R-free	0.16560
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.557
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.450	1.864
High resolution limit [Å]	1.800	1.800
Rmerge	0.078	0.569
Rmeas	0.080	0.579
Rpim	0.015	0.111
Number of reflections	25088	2434
<I/σ(I)>	6.84	1.3
Completeness [%]	99.9	99.88
Redundancy	10	4
CC(1/2)	1.000	0.991

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	291	1.6-1.9 M MgCl2 and 0.1 M Bicine pH 9.0