8KFD
Ferritin drug carrier(FDC) for encapsulated platinum (IV) prodrug for esophageal squamous cell carcinoma targeted therapy
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 183.450, 183.450, 183.450 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.300 - 1.800 |
| R-factor | 0.154 |
| Rwork | 0.153 |
| R-free | 0.16560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.557 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.450 | 1.864 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.078 | 0.569 |
| Rmeas | 0.080 | 0.579 |
| Rpim | 0.015 | 0.111 |
| Number of reflections | 25088 | 2434 |
| <I/σ(I)> | 6.84 | 1.3 |
| Completeness [%] | 99.9 | 99.88 |
| Redundancy | 10 | 4 |
| CC(1/2) | 1.000 | 0.991 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 291 | 1.6-1.9 M MgCl2 and 0.1 M Bicine pH 9.0 |
