8KEM
PKS domains-fused AmpC EC2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-03-16 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.98 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 108.470, 248.860, 153.700 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.369 - 2.770 |
R-factor | 0.230729608144 |
Rwork | 0.229 |
R-free | 0.26552 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.609 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | phenix.refine (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.840 |
High resolution limit [Å] | 2.770 | 2.770 |
Number of reflections | 53091 | 3749 |
<I/σ(I)> | 15.6 | |
Completeness [%] | 99.8 | |
Redundancy | 13.9 | |
CC(1/2) | 0.999 | 0.753 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 295 | 20mM MgCl2, 100mM sodium citrate/citric acid, and 40% polyethylene glycol 400 |