8KDL

Crystal structure of LmbF in complex with PLP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-14
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.1
Spacegroup name	P 1 21 1
Unit cell lengths	67.277, 74.017, 74.843
Unit cell angles	90.00, 93.18, 90.00

Refinement procedure

Resolution	48.630 - 1.700
R-factor	0.1658
Rwork	0.165
R-free	0.20400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.979
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.630	1.730
High resolution limit [Å]	1.700	1.700
Rmerge	0.113	0.488
Number of reflections	80638	4199
<I/σ(I)>	11.8	3
Completeness [%]	99.9	98.7
Redundancy	7
CC(1/2)	0.997	0.841

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M MES pH 6.5, 27% PEG 4000, 0.8M LiCl