8KC8
De novo design protein -T11
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-01-12 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | I 41 3 2 |
| Unit cell lengths | 150.212, 150.212, 150.212 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.500 - 2.650 |
| R-factor | 0.2247 |
| Rwork | 0.221 |
| R-free | 0.25530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.617 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.320 | 2.800 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Number of reflections | 8196 | 1007 |
| <I/σ(I)> | 35 | |
| Completeness [%] | 94.0 | |
| Redundancy | 61.8 | |
| CC(1/2) | 0.999 | 0.805 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 289 | 1.8M Sodium phosphate monobasic monohydrate,Potassium phosphate dibasic ,pH8.2 |
