8KBI
Structure of apo-AcrIIA7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-12-23 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 91.039, 91.039, 99.181 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.710 - 1.700 |
R-factor | 0.2104 |
Rwork | 0.210 |
R-free | 0.22680 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.517 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.710 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmeas | 0.280 | 1.886 |
Number of reflections | 101128 | 5207 |
<I/σ(I)> | 7.8 | 2.6 |
Completeness [%] | 100.0 | 100 |
Redundancy | 39.9 | 40 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 1.0 M Lithium chloride, 20% w/v Polyethylene glycol 6000 |