8KAR
Glutamate dehydrogenase-AKG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-11-11 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 95.267, 156.419, 99.330 |
Unit cell angles | 90.00, 110.30, 90.00 |
Refinement procedure
Resolution | 47.210 - 1.730 |
R-factor | 0.19826 |
Rwork | 0.197 |
R-free | 0.23065 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.595 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.210 | 1.760 |
High resolution limit [Å] | 1.730 | 1.730 |
Rmerge | 0.143 | 0.933 |
Rmeas | 0.156 | 1.034 |
Rpim | 0.060 | 0.437 |
Total number of observations | 37214 | |
Number of reflections | 141497 | 6919 |
<I/σ(I)> | 7.8 | 1.6 |
Completeness [%] | 100.0 | |
Redundancy | 6.7 | 5.4 |
CC(1/2) | 0.996 | 0.789 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM MES buffer (pH 6.5), 10% (w/v) PEG5,000 MME, 12% (v/v) 1-propanol, and 10% (v/v) glycerol |