8KAD

Crystal structure of an antibody light chain tetramer with 3D domain swapping

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-31
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	P 65 2 2
Unit cell lengths	52.780, 52.780, 260.290
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.700 - 2.000
R-factor	0.2554
Rwork	0.253
R-free	0.29570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.159
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.710	2.050
High resolution limit [Å]	2.000	2.000
Number of reflections	15551	1103
<I/σ(I)>	2.77
Completeness [%]	99.9
Redundancy	8.9
CC(1/2)	0.952	0.563

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.1M potassium sodium tartrate tetrahydrate