8K9Z
Crystal structure of Vibrio vulnificus RID-dependent transforming NADase domain (RDTND)/calmodulin-binding domain of Rho inactivation domain (RID-CBD) complexed with Ca2+-bound calmodulin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-02-24 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.9797 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 243.374, 49.778, 135.746 |
Unit cell angles | 90.00, 119.35, 90.00 |
Refinement procedure
Resolution | 48.510 - 2.900 |
R-factor | 0.26699 |
Rwork | 0.265 |
R-free | 0.30408 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | in-house model |
RMSD bond length | 0.016 |
RMSD bond angle | 1.102 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0415) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.950 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 31976 | 1543 |
<I/σ(I)> | 9.3 | |
Completeness [%] | 99.3 | |
Redundancy | 6.3 | |
CC(1/2) | 0.995 | 0.581 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 17% (w/v) PEG 3350 and 0.18 M magnesium acetate |